bipolaris species associated with rice plant: pathogenicity and genetic diversity of bipolaris oryzae using rep-pcr in mazandaran province of iran

نویسندگان

somayeh nazari

mohammad javan-nikkhah

khalil-berdi fotouhifar

vahid khosravi

alireza alizadeh

چکیده

ninety one monoconidial bipolaris isolates were obtained from lesions on different parts of rice in different locations of mazandaran province during the summer of 2009. bipolaris species were identified using morphological features such as color and shape of colony and color and size of conidia and conidiophores. the isolates were separated into two species; 85 (93.4%) isolates belonged to bipolaris oryzae and the remaining 6 (6.6%) isolates to bipolaris cynodontis. therefore b. oryzae is regarded as the major cause of rice brown spot disease in mazandaran province. in order to analyze genetic diversity among b. oryzae isolates, 71 isolates were subjected to fingerprinting analysis by rep-pcr using box and rep primers. in cluster analysis, 15 clonal lineages and 54 haplotypes were identified. the largest clonal lineage contained with 36 haplotypes was the most common lineage. these results also indicate a relatively high level of genetic diversity among b. oryzae isolates. also, pathogenicity test of a few b. oryzae isolates (12 isolates) was conducted under greenhouse condition and showed that those isolates were pathogenic to rice seedlings of cv. tarom. all isolates produced some leaf spots 24 h after inoculation.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

phylogeography and genetic diversity of the lesser mouse- eared bat (myotis blythii) in iran

in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...

Identification of some grass-associated species of Bipolaris, Curvularia and Exserohilum in selected regions of Iran

Infected tissues of roots and leaves of various grasses as well as soil around their rhizosphere were sampled from Fars, Golestan, Isfahan, Khorasan Shomali, Khorasan Razavi, Mazandaran and Semnan provinces (Iran). Based on morphological characteristics and available keys, four species of Bipolaris including B. austreliensis, B. indica, B. micropus and B. spicifera, four species of Curvularia i...

متن کامل

Genetic diversity of Fusarium semitectum isolates from rice, using RAPD and REP-PCR markers

In order to determine genetic variability among 33 isolates of Fusarium semitectum, morpho- logical and molecular studies were carried out. Isolates from different paddy fields in the north of Iran were separated from each other in four morphotype groups. The isolates showed a high level of variation in aerial mycelium, color of colony, number of septa and growth rate on potato dextrose agar (P...

متن کامل

occurence of bipolaris hawaiiensis associated with rice grain in iran

rice plant is one of the most important and strategic agricultural product with having highest cultivation area after wheat in the world. several factors including plant pathogenic fungi are causing agents of quantitative and qualitative losses in this plant. brown spot is an important seed borne disease of rice that caused by different species of bipolaris. brown spots observed on infected gra...

متن کامل

Control of brown spot pathogen of rice (Bipolaris oryzae) using some phenolic antioxidants

Bipolaris oryzae is the causal agent of rice brown spot disease and is responsible for significant economic losses. In order to control this disease, three phenolic antioxidants were tested (salicylic acid, benzoic acid and hydroquinone). The antifungal activity of the tested substances were investigated against B. oryzae at different concentrations in vitro, as well as the efficacy of their ex...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of crop protection

ناشر: tarbiat modares university

ISSN 2251-9041

دوره 4

شماره 4 2015

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023